Contact an Account ManagerSensitive method for the determination of chloroquine and its metabolite desethylchloroquine in human plasma and urine by HPLC
Summary
Type: HPLC
Application #: 104270
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 340nm, fluorescence, excitation 335nm, emission 370nm
Conditions: eluent: acetonitrile - phosphate buffer (40:60), ionic strength 0.1, pH 3, 75mM perchlorate
flow rate 0.8 - 1.0ml/min
Authors: Bergqvist Y
Source: J Chromatogr 221 (1980) 119 - 127
Details
Legend: Fig. 5. Chromatograms of plasma and urine samples obtained from a patient, UM =
unidentified metabolite, D = internal standard; left: plasma, 82nmol/l CQ,
16nmol/l CQM, fluorescence; middle: urine,